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Please use this identifier to cite or link to this item: https://wslhd.intersearch.com.au/wslhdjspui/handle/1/9011
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dc.contributor.authorVettori, M.-
dc.contributor.authorCarpene, G.-
dc.contributor.authorSalvagno, G. L.-
dc.contributor.authorGelati, M.-
dc.contributor.authorDima, F.-
dc.contributor.authorCelegon, G.-
dc.contributor.authorFavaloro, Emmanuel J.-
dc.contributor.authorLippi, G.-
dc.date.accessioned2024-03-11T00:58:02Z-
dc.date.available2024-03-11T00:58:02Z-
dc.date.issued2024-
dc.identifier.citationSeminars in Thrombosis & Hemostasis 50(2):275-283, 2024-
dc.identifier.urihttps://wslhd.intersearch.com.au/wslhdjspui/handle/1/9011-
dc.description.abstractPlatelets are central elements of hemostasis and also play a pivotal role in the pathogenesis of thrombosis in coronavirus disease 2019. This study was planned to investigate the effects of different severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) recombinant spike protein variants on platelet morphology and activation. Citrated whole blood collected from ostensibly healthy subjects was challenged with saline (control sample) and with 2 and 20 ng/mL final concentration of SARS-CoV-2 recombinant spike protein of Ancestral, Alpha, Delta, and Omicron variants. Platelet count was found to be decreased with all SARS-CoV-2 recombinant spike protein variants and concentrations tested, achieving the lowest values with 20 ng/mL Delta recombinant spike protein. The mean platelet volume increased in all samples irrespective of SARS-CoV-2 recombinant spike protein variants and concentrations tested, but especially using Delta and Alpha recombinant spike proteins. The values of both platelet function analyzer-200 collagen-adenosine diphosphate and collagen-epinephrine increased in all samples irrespective of SARS-CoV-2 recombinant spike protein variants and concentrations tested, and thus reflecting platelet exhaustion, and displaying again higher increases with Delta and Alpha recombinant spike proteins. Most samples where SARS-CoV-2 recombinant spike proteins were added were flagged as containing platelet clumps. Morphological analysis revealed the presence of a considerable number of activated platelets, platelet clumps, platelet-monocyte, and platelet-neutrophils aggregates, especially in samples spiked with Alpha and Delta recombinant spike proteins at 20 ng/mL. These results provide support to the evidence that SARS-CoV-2 is capable of activating platelets through its spike protein, though such effect varies depending on different spike protein variants. Copyright Thieme. All rights reserved.-
dc.titleEffects of Recombinant SARS-CoV-2 Spike Protein Variants on Platelet Morphology and Activation-
dc.typeJournal Article-
dc.identifier.doihttps://dx.doi.org/10.1055/s-0043-1769939-
dc.subject.keywordsSpike Glycoprotein, Coronavirus-
dc.subject.keywordsCovid-19-
dc.subject.keywordsCollagen-
dc.identifier.journaltitleSeminars in Thrombosis & Hemostasis-
dc.contributor.wslhdFavaloro, Emmanuel J.-
dc.identifier.pmid37327884-
dc.identifier.facilityWestmead-
Appears in Collections:Westmead Hospital 2019 - 2024

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